ATCC -30889™

平台编号：bio-48035
拉丁名:
具体用途:Zymodeme II.
规格:test tube
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ATCC® Number:30889™
Organism: Entamoeba histolytica Schaudinn
Designations: H-458:CDC [ATCC30217]
Isolation: feces from human adult female with amebic dysentery, Asia (?), (patient in U.S. for treatment), 1971
Depositors: LS Diamond
History: ATCC<<--LS Diamond<<--G.R. Healy
Biosafety Level:2
Shipped: test tube
Growth Conditions: ATCCmedium 2463: LYI-S-2 medium, modifiedTemperature: 35.0°C Duration: axenic; anaerobic Protocol: ATCCNO: 30016 SPEC: To subculture: Chill the parent culture in an ice bath for 5-10 minutes, suspend the organisms by inverting the tube 20 times, then transfer 0.1-0.5 ml of the suspension to fresh medium in a glass tube. Maintain parallel cultures at different inoculation levels. Cap the tubes tightly and maintain them in a horizontal position. Use only borosilicate glass tubes. Depending on its source, plasticware may not support growth, or at best may not support growth equivalent to that in glass.
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Comments: Zymodeme II.
Classification: KINGDOM: Protozoa
References: 4847: Mattern CF, Keister DB. Experimental amebiasis. II. Hepatic amebiasis in the newborn hamster. Am. J. Trop. Med. Hyg. 26: 402-411, 1977. PubMed: 194492 23839: Diamond LS, et al. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans. R. Soc. Trop. Med. Hyg. 72: 431-432, 1978. PubMed: 212851